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Kerala develops RT-PCR kit to detect range of mutant Covid strains: All you need to know

India city Pune covid-19 RNA isolate
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RT-PCR kit at the National Institute of Virology in Pune and found it satisfactory.The new multiplex RT-PCR kit targets two SARS-CoV-2 genes -- RdRp and ORFb-nsp14 -- and the human RNAse P gene as the internal control to help detect a range of mutant strains.The ORFb-nsp14 is one of the least mutated genes in Covid-19 and currently, there are no kits in the market with ORF-nsp14 as the target.The new kit is based on multiplex Taqman chemistry, amplifying all three genes in a single reaction.

The amplification time for the assay is 45 minutes, apart from the time required for the RNA isolation from nasopharyngeal swab samples.Multiplexing two confirmatory genes will help short-list possible new variants if one of the genes fails to amplify.

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Covid-19.rehab - the latest news concerning the spread of the SARS-CoV-2 coronavirus (formerly 2019-nCoV), as well as the infectious disease that it causes COVID-19, in separate countries and in the world as a whole. Current news on the topic of coronavirus SARS-CoV-2. Quarantine, pandemic 2020, epidemic: what is it, when will it come to end, precautions and how to protect yourself from this disease. The most honest news, no fakes! To keep up with the latest news, stay tuned for updates on the site! Every day we will collect news and statistics on coronavirus from around the world for you to be aware of this topic.

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